pRSETB-ro4GFP
(Plasmid
#82368)
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PurposeInducible expression of redox sensitive GFP in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSETB
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namero4GFP
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Speciesjellyfish
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Insert Size (bp)714
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MutationC48S/S65T/Q80R/N149C/S202C
- Promoter T7
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Tag
/ Fusion Protein
- 6xHIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoger Tsien, UCSD
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETB-ro4GFP was a gift from S. James Remington (Addgene plasmid # 82368 ; http://n2t.net/addgene:82368 ; RRID:Addgene_82368) -
For your References section:
Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, Remington SJ. J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13. 10.1074/jbc.M312846200 PubMed 14722062