TU#1448_unc-73b_cDNA
(Plasmid
#82362)
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Purposeexpress unc-73b cDNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR-221
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameunc-73 b isoform
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SpeciesC. elegans (nematode)
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Insert Size (bp)4917
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Entrez Geneunc-73 (a.k.a. CELE_F55C7.7)
- Promoter none
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TU#1448_unc-73b_cDNA was a gift from Martin Chalfie (Addgene plasmid # 82362 ; http://n2t.net/addgene:82362 ; RRID:Addgene_82362) -
For your References section:
GEFs and Rac GTPases control directional specificity of neurite extension along the anterior-posterior axis. Zheng C, Diaz-Cuadros M, Chalfie M. Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6973-8. doi: 10.1073/pnas.1607179113. Epub 2016 Jun 6. 10.1073/pnas.1607179113 PubMed 27274054