pVHed07
(Plasmid
#82350)
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PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneR6K
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 4990
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir2+
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namemKate2 RFP
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Insert Size (bp)696
Gene/Insert 2
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Gene/Insert nameSuperfolder GFP
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Insert Size (bp)720
Gene/Insert 3
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Gene/Insert nameTemporal logic gate with TP901 and Bxb1 attachment sites
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Insert Size (bp)394
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid has a R6K ori requires a Pir2+ strain to replicate. When this plasmid is co-transformed with pAH123 (Addgene #66077) and grown at 37, the plasmid is linearized and integrated into the Phi80 site on the E. coli chromosome. The use of the R6K origin ensures that non-integrated plasmids will not replicate in the target strain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVHed07 was a gift from Richard Murray (Addgene plasmid # 82350 ; http://n2t.net/addgene:82350 ; RRID:Addgene_82350) -
For your References section:
A population-based temporal logic gate for timing and recording chemical events. Hsiao V, Hori Y, Rothemund PW, Murray RM. Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663. PubMed 27193783