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pRS314-U6-G96C/A97C/U100C/U101C
(Plasmid #82328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4800
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6 RNA with G96C/A97C/U100C/U101C mutation
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    300
  • Mutation
    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C)
  • GenBank ID
    NR_132206.1
  • Entrez Gene
    snR6 (a.k.a. snR6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Burke, Butcher, and Brow. RNA. (2015)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS314-U6-G96C/A97C/U100C/U101C was a gift from Aaron Hoskins (Addgene plasmid # 82328 ; http://n2t.net/addgene:82328 ; RRID:Addgene_82328)

  • For your References section:

    A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex. Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA. Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 10.1093/nar/gkw686. Epub 2016 Aug 2. 10.1093/nar/gkw686 PubMed 27484481
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