pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A
(Plasmid
#82176)
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PurposeGateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection. (Please note this plasmid contains S45A mutation, not T45A)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2790
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Vector typeGateway Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTNNB1
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SpeciesH. sapiens (human)
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MutationS33A, S37A, T41A, S45A
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GenBank IDNM_001904.3
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Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Closed state: stop codon immediately follows insert Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82176 ; http://n2t.net/addgene:82176 ; RRID:Addgene_82176) -
For your References section:
Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. 10.1158/2159-8290.CD-16-0160 PubMed 27147599