pPA-RL-FL
(Plasmid
#78863)
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PurposeDULIP Tandem Construct used for data normalization
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5428
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Modifications to backboneProtein A, Renilla luciferase, Firefly luciferase inserted via LR reaction
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Vector typeMammalian Expression ; Expression vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFirefly
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SpeciesPhoturis lucicrescens
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Insert Size (bp)1650
- Promoter CMV
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Tag
/ Fusion Protein
- Protein A, Renilla luciferase (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVF_pcDNA3, T7F
- 3′ sequencing primer bGH reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPA-RL-FL was a gift from Erich Wanker (Addgene plasmid # 78863 ; http://n2t.net/addgene:78863 ; RRID:Addgene_78863) -
For your References section:
DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells. Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE. J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. 10.1016/j.jmb.2015.08.003 PubMed 26264872