pdCas9 (GB1079)
(Plasmid #75399)

• Purpose: Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
• Depositing Lab: Diego Orzaez
• Publication: Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: self-made; derived from pGEMT-Easy manufactured by Promega
• Backbone size w/o insert (bp): 3055
• Total vector size (bp): 7192
• Vector type: Plant Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
• Species: Streptococcus pyogenes
• Insert Size (bp): 4137

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry