pCfB1975 (pUC19-PTEF1-PPGK1)
(Plasmid
#73296)
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PurposeExample plasmid containing a bidirectional promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 4117
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Modifications to backboneLacZ removed. Bidirectional promoters pTEF1 and pPGK1 inserted.
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepTEF1-pPGK1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1837
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene verified sequence differs from the depositor's reference map. The TEF and PPGK promoter region is flipped in comparison.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCfB1975 (pUC19-PTEF1-PPGK1) was a gift from Irina Borodina (Addgene plasmid # 73296 ; http://n2t.net/addgene:73296 ; RRID:Addgene_73296) -
For your References section:
EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, Keasling JD, Borodina I. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23. 10.1002/biot.201600147 PubMed 27166612