Skip to main content
Addgene

RIP7-RLuc-YFP-pBS
(Plasmid #72482)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72482 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 13000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    rat ins2 gene promoter
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    9890
  • Entrez Gene
    Ins2 (a.k.a. CP-II)

Gene/Insert 2

  • Gene/Insert name
    luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    1101
  • Tag / Fusion Protein
    • eYFP (C terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    eYFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    784
  • Mutation
    eYFP varient
  • Tag / Fusion Protein
    • renilla luciferase (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RIP7-pBS: Dr. Thomas Kay (St. Vincent Institute, Melbourne Australia)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RIP7-RLuc-YFP-pBS was a gift from Vincent Poitout (Addgene plasmid # 72482 ; http://n2t.net/addgene:72482 ; RRID:Addgene_72482)
  • For your References section:

    Dual reporter-beta-cell specific male transgenic rats for the analysis of beta-cell functional mass and enrichment by flow cytometry. Ghislain J, Fontes G, Tremblay C, Kebede MA, Poitout V. Endocrinology. 2015 Dec 15:en20151550. 10.1210/en.2015-1550 PubMed 26671180