RIP7-RLuc-YFP-pBS
(Plasmid
#72482)
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PurposeExpresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 13000
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namerat ins2 gene promoter
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SpeciesR. norvegicus (rat)
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Insert Size (bp)9890
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Entrez GeneIns2 (a.k.a. CP-II)
Gene/Insert 2
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Gene/Insert nameluciferase
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SpeciesRenilla reniformis
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Insert Size (bp)1101
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Tag
/ Fusion Protein
- eYFP (C terminal on insert)
Gene/Insert 3
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Gene/Insert nameeYFP
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SpeciesAequorea victoria
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Insert Size (bp)784
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MutationeYFP varient
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Tag
/ Fusion Protein
- renilla luciferase (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRIP7-pBS: Dr. Thomas Kay (St. Vincent Institute, Melbourne Australia)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RIP7-RLuc-YFP-pBS was a gift from Vincent Poitout (Addgene plasmid # 72482 ; http://n2t.net/addgene:72482 ; RRID:Addgene_72482) -
For your References section:
Dual reporter-beta-cell specific male transgenic rats for the analysis of beta-cell functional mass and enrichment by flow cytometry. Ghislain J, Fontes G, Tremblay C, Kebede MA, Poitout V. Endocrinology. 2015 Dec 15:en20151550. 10.1210/en.2015-1550 PubMed 26671180