pJK573
(Plasmid
#72456)
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PurposeProduces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 3616
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN5-carboxyaminoimidazole ribonucleotide mutase
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Alt namepurE
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Alt nameYPO3076
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SpeciesYersinia pestis strain CO92
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Mutationsynthetic gene with ~57 silent substitutions
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GenBank IDYP_002347996.1
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- TEV cleavage site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Database annotation "phosphoribosylaminoimidazole carboxylase catalyic subuni" is incorrect.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK573 was a gift from T. J. Kappock (Addgene plasmid # 72456 ; http://n2t.net/addgene:72456 ; RRID:Addgene_72456)