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Addgene

pJK573
(Plasmid #72456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3616
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide mutase
  • Alt name
    purE
  • Alt name
    YPO3076
  • Species
    Yersinia pestis strain CO92
  • Mutation
    synthetic gene with ~57 silent substitutions
  • GenBank ID
    YP_002347996.1
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Database annotation "phosphoribosylaminoimidazole carboxylase catalyic subuni" is incorrect.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK573 was a gift from T. J. Kappock (Addgene plasmid # 72456 ; http://n2t.net/addgene:72456 ; RRID:Addgene_72456)
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