pCAG-HDAC4*
(Plasmid
#71402)
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PurposeCAG promoter, mammalian expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGEN
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Backbone manufacturerAddgene plasmid #11160
- Backbone size w/o insert (bp) 4798
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDAC4*
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SpeciesH. sapiens (human)
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Insert Size (bp)3400
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MutationAfter aa126, insertion leads to reading frame shift and early termination.
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GenBank IDNM_006037.3
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Entrez GeneHDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-HDAC4* was a gift from Bo Chen (Addgene plasmid # 71402 ; http://n2t.net/addgene:71402 ; RRID:Addgene_71402) -
For your References section:
A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa. Guo X, Wang SB, Xu H, Ribic A, Mohns EJ, Zhou Y, Zhu X, Biederer T, Crair MC, Chen B. Nat Commun. 2015 Aug 14;6:8005. doi: 10.1038/ncomms9005. 10.1038/ncomms9005 PubMed 26272629
Map uploaded by the depositor.