pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR
(Plasmid
#67931)
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PurposeTranslational reporter - ARHGAP5 UTRs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP-NLS-P2A-NLS-mCherry
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SpeciesH. sapiens (human)
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Entrez GeneARHGAP5 (a.k.a. GFI2, RhoGAP5, p190-B, p190BRhoGAP)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR was a gift from Ron Vale (Addgene plasmid # 67931 ; http://n2t.net/addgene:67931 ; RRID:Addgene_67931) -
For your References section:
Regulation of mRNA translation during mitosis. Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD. Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957. 10.7554/eLife.07957 PubMed 26305499