pHR-mCherry-Emi1_ARHGAP5 UTRs
(Plasmid
#67927)
-
PurposeExpresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 67927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCherry-Emi1
-
Alt nameFBOX5
-
Alt nameARHGAP5 UTR
-
SpeciesH. sapiens (human)
-
Entrez GeneFBXO5 (a.k.a. EMI1, FBX5, Fbxo31)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pHR-SIN-F (5'-CCAAGGACCTGAAATGACCC-3')
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-mCherry-Emi1_ARHGAP5 UTRs was a gift from Ron Vale (Addgene plasmid # 67927 ; http://n2t.net/addgene:67927 ; RRID:Addgene_67927) -
For your References section:
Regulation of mRNA translation during mitosis. Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD. Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957. 10.7554/eLife.07957 PubMed 26305499