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PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehGtACR1
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Alt nameGtACR1
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Alt nameGuillardia theta anion channelrhodopsin (ACR)
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SpeciesGuillardia theta
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Insert Size (bp)1613
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site, in the pFUGW vector backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFUGW-hGtACR1-EYFP was a gift from John Spudich (Addgene plasmid # 67795 ; http://n2t.net/addgene:67795 ; RRID:Addgene_67795) -
For your References section:
Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL. Science. 2015 Jun 25. pii: aaa7484. 10.1126/science.aaa7484 PubMed 26113638