pcDNA3.1-Flag-ZFP809(1-353)
(Plasmid
#67788)
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PurposeMammalian expression of 1-353 amino acids of ZFP809 tagged with Flag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZFP809
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SpeciesM. musculus (mouse)
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Entrez GeneZfp809 (a.k.a. BB114266, C330026E23)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Flag-ZFP809(1-353) was a gift from Stephen Goff (Addgene plasmid # 67788 ; http://n2t.net/addgene:67788 ; RRID:Addgene_67788) -
For your References section:
Embryonic stem cells use ZFP809 to silence retroviral DNAs. Wolf D, Goff SP. Nature. 2009 Apr 30;458(7242):1201-4. doi: 10.1038/nature07844. Epub 2009 Mar 8. 10.1038/nature07844 PubMed 19270682