pHKMC1: Empty sgRNA for Cloning
(Plasmid
#67720)
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Purpose(Empty Backbone) Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneU6::unc-119_sgRNA from J.Calarco (Plasmid #46169)
- Backbone size (bp) 3479
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Modifications to backboneunc-119 sgRNA sequence from PU6::unc-119_sgRNA(Plasmid #46169) was replaced by NotI and BamHI sites for sgRNA cloning purpose.
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Vector typeWorm Expression
- Promoter PU6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsNEB 5-alpha (DH5alpha derivative)
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHKMC1: Empty sgRNA for Cloning was a gift from Monica Colaiacovo (Addgene plasmid # 67720 ; http://n2t.net/addgene:67720 ; RRID:Addgene_67720) -
For your References section:
Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes. Norris AD, Kim HM, Colaiacovo MP, Calarco JA. Genetics. 2015 Oct;201(2):449-58. doi: 10.1534/genetics.115.180679. Epub 2015 Jul 30. 10.1534/genetics.115.180679 PubMed 26232410
