pUNC2001
(Plasmid
#67280)
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PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMalc2
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVibrio cholera photolyase
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SpeciesVibrio cholera
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Insert Size (bp)2000
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Mutationnone known
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GenBank IDNC_002505.1
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Tag
/ Fusion Protein
- maltose binding protein (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer MBP-F
- 3′ sequencing primer pTrcHis-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUNC2001 was a gift from Aziz Sancar (Addgene plasmid # 67280 ; http://n2t.net/addgene:67280 ; RRID:Addgene_67280) -
For your References section:
Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from Vibrio cholerae. Worthington EN, Kavakli IH, Berrocal-Tito G, Bondo BE, Sancar A. J Biol Chem. 2003 Oct 3;278(40):39143-54. Epub 2003 Jul 22. 10.1074/jbc.M305792200 PubMed 12878596