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PurposeMammalian expression of SUMO2 with a strep and an HA tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 5823
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUMO2
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Alt namesmall ubiquitin-like modifier 2
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SpeciesH. sapiens (human)
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Insert Size (bp)450
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GenBank IDAAH08450.1
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Entrez GeneSUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
- Promoter CMV
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Tags
/ Fusion Proteins
- Strep (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV For
- 3′ sequencing primer BGH Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-StrepHA-SUMO2 was a gift from Ying Liu (Addgene plasmid # 66867 ; http://n2t.net/addgene:66867 ; RRID:Addgene_66867) -
For your References section:
Regulation of SUMO2 target proteins by the proteasome in human cells exposed to replication stress. Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y. J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. Epub 2015 Mar 19. 10.1021/pr500997p PubMed 25748227