CmCitrine Lck w/ NosUTRs
(Plasmid
#66785)
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Purposeexpression of fluorescent membranes in sea urchin primordial germ cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typesea urchin, zebrafish, xenopus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLck
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Alt nameLCK proto-oncogene, Src family tyrosine kinase
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SpeciesH. sapiens (human)
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Insert Size (bp)2144
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MutationTandem repeat of LCK the N-terminal membrane targeting sequence: MGCIKSKRKDNLNDDEAAMGCIKSKRKDNLNDDEAPVAT
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Entrez GeneLCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
- Promoter T7
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Tags
/ Fusion Proteins
- 5' UTR from sea urchin Nanos (N terminal on insert)
- mCitrine (C terminal on insert)
- 3' UTR from sea urchin Nanos (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byLck was taken from Addgene plasmid #53749
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The membrane anchoring of mCitrine in sea urchin germ cells uses the sea urchin (S. purpuratus) 5' and 3' UTRs Nanos2 UTRs flanking this insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CmCitrine Lck w/ NosUTRs was a gift from Amro Hamdoun (Addgene plasmid # 66785 ; http://n2t.net/addgene:66785 ; RRID:Addgene_66785) -
For your References section:
Migration of sea urchin primordial germ cells. Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun A. Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 Apr 30. 10.1002/dvdy.24133 PubMed 24677545