pCVD531
(Plasmid
#66160)
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PurposeA 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned into the HindIII site of pBR322.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdh-like
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Insert Size (bp)8900
- Promoter Unknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBRforEco (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned from the chromosome into the HindIII site of pBR322.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD531 was a gift from James Kaper (Addgene plasmid # 66160 ; http://n2t.net/addgene:66160 ; RRID:Addgene_66160) -
For your References section:
Comparative analysis of the hemolysin genes of Vibrio cholerae non-01, V. mimicus, and V. hollisae that are similar to the tdh gene of V. parahaemolyticus. Nishibuchi M, Khaeomanee-iam V, Honda T, Kaper JB, Miwatani T. FEMS Microbiol Lett. 1990 Feb;55(3):251-6. PubMed 2323548