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Addgene

pCVD529
(Plasmid #66159)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdh2
  • Insert Size (bp)
    2700
  • Promoter Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBRforEco
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD529 was a gift from James Kaper (Addgene plasmid # 66159 ; http://n2t.net/addgene:66159 ; RRID:Addgene_66159)

  • For your References section:

    Duplication and variation of the thermostable direct haemolysin (tdh) gene in Vibrio parahaemolyticus. Nishibuchi M, Kaper JB. Mol Microbiol. 1990 Jan;4(1):87-99. PubMed 2319944
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