pAH123
(Plasmid
#66077)
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PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAH123
- Total vector size (bp) 5192
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namephi80 integrase
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Insert Size (bp)1251
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAH123 was a gift from Tom Ellis (Addgene plasmid # 66077 ; http://n2t.net/addgene:66077 ; RRID:Addgene_66077) -
For your References section:
Quantifying cellular capacity identifies gene expression designs with reduced burden. Ceroni F, Algar R, Stan GB, Ellis T. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6. 10.1038/nmeth.3339 PubMed 25849635
Map uploaded by the depositor.
