AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
(Plasmid
#65429)
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PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerStratagene
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Modifications to backboneA fragment containing: the hSyn1 promoter-a (Rox-Stop-Rox) cassette-a FLEX cassette with Lox71/LoxJTZ17 sites flanking (ChR2H134R-EYFP)-WPRE-bGHpA were swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 4439 bp.
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChR2(H134R)-EYFP
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Alt namechannelrhodopsin-2 variant H134R-EYFP
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SpeciesC. reinhardtii
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Insert Size (bp)1671
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MutationH134R variant
- Promoter hSyn1
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (destroyed during cloning)
- 3′ cloning site Not1 (destroyed during cloning)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA was a gift from Hongkui Zeng (Addgene plasmid # 65429 ; http://n2t.net/addgene:65429 ; RRID:Addgene_65429) -
For your References section:
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H. Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. 10.1016/j.neuron.2015.02.022 PubMed 25741722