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PurposeAAV-mediated expression of Jaws-ER2 under the human synapsin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4695
- Total vector size (bp) 6351
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Growth instructionsPlease note that this backbone is highly recombination prone and should be amplified in a rec- strain like Sure2 or Stbl3, and grown at 30 C using carbenicillin rather than ampicillin.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameJaws-GFP-ER2
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Alt nameJaws-ER2
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SpeciesHaloarcula salinarum (strain Shark)
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Insert Size (bp)1584
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MutationK200R W214F
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GenBank IDKM000927.1
- Promoter human synapsin
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Tags
/ Fusion Proteins
- GFP (C terminal on insert)
- ER2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GCG AGG CGC GAG ATA G
- 3′ sequencing primer CAA GGA GGA GAA AAT GAA AGC C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hsyn-Jaws-GFP-ER2 was a gift from Edward Boyden (Addgene plasmid # 65016 ; http://n2t.net/addgene:65016 ; RRID:Addgene_65016) -
For your References section:
Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES. Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6. 10.1038/nn.3752 PubMed 24997763