pLH-stsgRNA2.1
(Plasmid #64117)

• Purpose: Vector for expression of St1 sgRNA2.1 in mammalian cells
• Depositing Lab: Thoru Pederson
• Publication: Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 8274
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: St1 sgRNA2.1
• gRNA/shRNA sequence: gtctttgtactctcaagagaaatcttgcagaagctacaaagataaggcttcatgccgaaatcaacaccctgtcattctatggcagggtg
• Species: Synthetic
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: cccttcaccgagggcctatttccc
• 3′ sequencing primer: CTATTCTTTCCCCTGCACTGTACCC

Resource Information

• Supplemental Documents:
  • A rapid sgRNA cloning protocol for Multicolor CRISPR
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry