pFusX3-6
(Plasmid
#63352)
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PurposeEncodes RVD positions 7-9 in TALEN assembly
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFusX3
- Backbone size w/o insert (bp) 3015
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNI HD HD
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Alt nameX3_ACC
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SpeciesSynthetic
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Mutationnone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer pCR8_F1
- 3′ sequencing primer pCR8_R1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFusX3-6 was a gift from Stephen Ekker (Addgene plasmid # 63352 ; http://n2t.net/addgene:63352 ; RRID:Addgene_63352) -
For your References section:
FusX: A rapid one-step TALE assembly system for genome science. Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC. Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172 10.1089/hum.2015.172 PubMed 26854857