RFN_EGFP_47
(Plasmid
#60071)
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PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSQT1313
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Backbone manufacturerJoung lab (Addgene plasmid # 53370)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepair of multiplex gRNAs targeting EGFP
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Alt nameEGFP gRNA pair #47
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Left gRNA target site: AAGTTCAGCGTGTCCGGCGA
Right gRNA target site: CCGTCCAGCTCGACCAGGAT
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RFN_EGFP_47 was a gift from Keith Joung (Addgene plasmid # 60071 ; http://n2t.net/addgene:60071 ; RRID:Addgene_60071) -
For your References section:
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325