-
PurposeExpress infrared fluorescent protein IFP2.0 plus HO1 enzyme
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 59427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLenti CMV/TO Puro DEST (670-1
-
Backbone manufactureraddgene
- Backbone size w/o insert (bp) 9594
-
Vector typeLentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl2
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameIFP2.0T2AHO1 IRES EGFP
-
Insert Size (bp)3203
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLenti CMV-IFP2.0 T2A HO1 was a gift from Xiaokun Shu (Addgene plasmid # 59427 ; http://n2t.net/addgene:59427 ; RRID:Addgene_59427) -
For your References section:
An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA, Shu X. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. 10.1038/ncomms4626 PubMed 24832154