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Addgene

pLentiPGK Blast DEST JNKKTRmRuby2
(Plasmid #59154)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59154 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLENTI PGK Blast DEST (w524-1)
  • Backbone manufacturer
    Eric Campeau Lab (Addgene Plasmid 19065)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    JNK Kinase Translocation Reporter
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPK8 (a.k.a. JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
  • Promoter PGK
  • Tag / Fusion Protein
    • mRuby2

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiPGK Blast DEST JNKKTRmRuby2 was a gift from Markus Covert (Addgene plasmid # 59154 ; http://n2t.net/addgene:59154 ; RRID:Addgene_59154)
  • For your References section:

    High-sensitivity measurements of multiple kinase activities in live single cells. Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW. Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039. 10.1016/j.cell.2014.04.039 PubMed 24949979