pCMV6-Entry IL36RN R102W
(Plasmid
#58323)
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Purposeentry vector for human IL36RN R102W mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6-entry
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 5300
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Vector typeEntry vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIL36RN
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SpeciesH. sapiens (human)
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MutationR102W: c.304C>T, p.Arg102Trp
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GenBank IDNM_012275.2
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Entrez GeneIL36RN (a.k.a. FIL1, FIL1(DELTA), FIL1D, IL-36Ra, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, PSORS14)
- Promoter CMV
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Tag
/ Fusion Protein
- myc-DDK (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SgfI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6-Entry IL36RN R102W was a gift from Francesca Capon (Addgene plasmid # 58323 ; http://n2t.net/addgene:58323 ; RRID:Addgene_58323) -
For your References section:
In Silico and In Vitro Analysis of IL36RN Alterations Reveals Critical Residues for the Function of the Interleukin-36 Receptor Complex. Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F. J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 10.1016/j.jid.2023.06.191. Epub 2023 Jul 4. 10.1016/j.jid.2023.06.191 PubMed 37414245