pSEVA12DKR-J
(Plasmid
#58306)
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PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSEVA
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsGrowth may be sluggish due to high expression levels of genomic cassette.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGenomic cassette
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byZeta/Epsilon. Toxin-Antitoxin parts. Part source: Dr. Urszula Zielenkiewicz (Dept of Microbial Biochemistry, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA12DKR-J was a gift from Tom Ellis (Addgene plasmid # 58306 ; http://n2t.net/addgene:58306 ; RRID:Addgene_58306) -
For your References section:
GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673