pGLTR-X-PURO
(Plasmid #58246)

• Purpose: GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection
• Depositing Lab: Stephan Geley
• Publication: Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.

Backbone

• Vector backbone: pGLTR-X-GFP
• Total vector size (bp): 12000
• Vector type: Mammalian Expression, Lentiviral, RNAi ; GATEWAY destionation vector
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Puro(R)
• Species: Streptomyces
• Insert Size (bp): 600
• Promoter: SFFV
• Tag / Fusion Protein:
  • TetR-NLS-T2A (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n.a.
• 3′ sequencing primer: n.a.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.