PLEXIN A1
(Plasmid
#58237)
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PurposeRiboprobe for in vitro transcription of Plexin A1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58237 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS KS+
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlexin A1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
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Entrez GenePlxna1 (a.k.a. 2600013D04Rik, NOV, PlexA1, Plxn1, mKIAA4053)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cDNA from expressed sequence tag (EST) IMAGE clones (Invitrogen), clone ID 1853022 (Plxna1).
For antisense RNA transcription, use EcoR1 and T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PLEXIN A1 was a gift from Christiana Ruhrberg (Addgene plasmid # 58237 ; http://n2t.net/addgene:58237 ; RRID:Addgene_58237) -
For your References section:
Semaphorin3A, Neuropilin-1, and PlexinA1 are required for lymphatic valve formation. Bouvree K, Brunet I, Del Toro R, Gordon E, Prahst C, Cristofaro B, Mathivet T, Xu Y, Soueid J, Fortuna V, Miura N, Aigrot MS, Maden CH, Ruhrberg C, Thomas JL, Eichmann A. Circ Res. 2012 Aug 3;111(4):437-45. doi: 10.1161/CIRCRESAHA.112.269316. Epub 2012 Jun 21. 10.1161/CIRCRESAHA.112.269316 PubMed 22723296