LII ins 2-3
(Plasmid
#54379)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAMP (Genart)
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Backbone manufacturerGeneart
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinsulator sequence, dummy 2-3
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LII/LIV dummy, contain 2 repeats of a palindromic 16 bp sequence. These nucleotides have been described to act as an insulator sequence in plants, thus preventing the influence of neighboring promoters on transcriptional activation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LII ins 2-3 was a gift from Martin Parniske (Addgene plasmid # 54379 ; http://n2t.net/addgene:54379 ; RRID:Addgene_54379) -
For your References section:
A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M. PLoS One. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218. eCollection 2014. 10.1371/journal.pone.0088218 PubMed 24551083