mEmerald EFHC2-N-18
(Plasmid
#54077)
-
PurposeLocalization: EF-Hand Domain , Excitation: 487, Emission: 509
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 54077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backbonemEmerald-N1
- Backbone size w/o insert (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEFHC2
-
Alt nameEF-hand domain (C-terminal)
-
Alt nameNM_025184.3
-
SpeciesH. sapiens (human)
-
GenBank ID
-
Entrez GeneEFHC2 (a.k.a. MRX74, dJ1158H2.1)
- Promoter CMV
-
Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EF-hand domain (C-terminal). Excitation = 487; Emission = 509. Localization data: EF-Hand Domain
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mEmerald EFHC2-N-18 was a gift from Michael Davidson (Addgene plasmid # 54077 ; http://n2t.net/addgene:54077 ; RRID:Addgene_54077)