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Addgene

pCR2.1-Ins2-842
(Plasmid #53969)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53969 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Backbone manufacturer
    Life Technologies
  • Total vector size (bp) 4787
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse Ins2 gene partial
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    842
  • GenBank ID
    NM_001185083.1
  • Entrez Gene
    Ins2 (a.k.a. Ins-2, InsII, Mody, Mody4)
  • Promoter lac

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1-Ins2-842 was a gift from Kevin Ferreri (Addgene plasmid # 53969 ; http://n2t.net/addgene:53969 ; RRID:Addgene_53969)
  • For your References section:

    Development of a quantitative methylation-specific polymerase chain reaction method for monitoring beta cell death in type 1 diabetes. Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K. PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub 2012 Oct 29. 10.1371/journal.pone.0047942 PubMed 23144715