pCR2.1-Ins2-842
(Plasmid
#53969)
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Purpose842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerLife Technologies
- Total vector size (bp) 4787
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemouse Ins2 gene partial
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SpeciesM. musculus (mouse)
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Insert Size (bp)842
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GenBank IDNM_001185083.1
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Entrez GeneIns2 (a.k.a. Ins-2, InsII, Mody, Mody4)
- Promoter lac
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1-Ins2-842 was a gift from Kevin Ferreri (Addgene plasmid # 53969 ; http://n2t.net/addgene:53969 ; RRID:Addgene_53969) -
For your References section:
Development of a quantitative methylation-specific polymerase chain reaction method for monitoring beta cell death in type 1 diabetes. Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K. PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub 2012 Oct 29. 10.1371/journal.pone.0047942 PubMed 23144715