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PurposeTranscription of guide RNA (gRNA) in wheat cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53062 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC-T
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Backbone manufacturerCWBIO
- Backbone size w/o insert (bp) 2700
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Vector typeCRISPR ; Plant expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namewheat U6 promoter - gRNA
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Specieswheat
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Insert Size (bp)475
- Promoter wheat U6
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer LacZ-F2
- 3′ sequencing primer ASK-FN2 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6-gRNA was a gift from Caixia Gao (Addgene plasmid # 53062 ; http://n2t.net/addgene:53062 ; RRID:Addgene_53062) -
For your References section:
Targeted genome modification of crop plants using a CRISPR-Cas system. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. 10.1038/nbt.2650 PubMed 23929338
Map uploaded by the depositor.
