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PurposeExpresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6749
- Total vector size (bp) 7400
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Modifications to backboneHis tag, linker, enzymatic biotinylation sequence, and rat CD4 d3+4 (HisLBio-CD4d3+4).
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD154
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Alt nameTumor necrosis factor ligand superfamily member 5
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SpeciesH. sapiens (human)
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Insert Size (bp)651
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Entrez GeneCD40LG (a.k.a. CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L)
- Promoter CMV
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Tags
/ Fusion Proteins
- His tag (N terminal on backbone)
- enzymatic biotinylation sequence (N terminal on backbone)
- rat CD4 d3+4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert was chemically synthesised.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Article ref 115
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CD154-His-bio was a gift from Gavin Wright (Addgene plasmid # 52337 ; http://n2t.net/addgene:52337 ; RRID:Addgene_52337) -
For your References section:
A human platelet receptor protein microarray identifies FcepsilonR1alpha as an activating PEAR1 ligand. Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright GJ. Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946. 10.1074/mcp.M114.046946 PubMed 25713122