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Addgene

EGR-1 TurboRFP
(Plasmid #52306)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52306 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTurboRFP-PRL
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 4127
  • Total vector size (bp) 4654
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGR-1 Promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    527
  • Entrez Gene
    Egr1 (a.k.a. A530045N19Rik, ETR103, Egr-1, Krox-1, Krox-24, Krox24, NGF1-A, NGFI-A, NGFIA, TIS8, Zenk, Zfp-6, Zif268, egr)
  • Tag / Fusion Protein
    • TurboRFP (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGR-1 TurboRFP was a gift from Joel Voldman (Addgene plasmid # 52306 ; http://n2t.net/addgene:52306 ; RRID:Addgene_52306)
  • For your References section:

    A cell-based sensor of fluid shear stress for microfluidics. Varma S, Voldman J. Lab Chip. 2015 Feb 4. 10.1039/c4lc01369g PubMed 25648195