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PurposeFluid Shear Stress inducible Red Fluorescent Protein for mammalian expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTurboRFP-PRL
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 4127
- Total vector size (bp) 4654
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGR-1 Promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)527
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Entrez GeneEgr1 (a.k.a. A530045N19Rik, ETR103, Egr-1, Krox-1, Krox-24, Krox24, NGF1-A, NGFI-A, NGFIA, TIS8, Zenk, Zfp-6, Zif268, egr)
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Tag
/ Fusion Protein
- TurboRFP (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGR-1 TurboRFP was a gift from Joel Voldman (Addgene plasmid # 52306 ; http://n2t.net/addgene:52306 ; RRID:Addgene_52306) -
For your References section:
A cell-based sensor of fluid shear stress for microfluidics. Varma S, Voldman J. Lab Chip. 2015 Feb 4. 10.1039/c4lc01369g PubMed 25648195