Split 'n coexpress CUL3-RBX1
(Plasmid #52293)

• Purpose: bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
• Depositing Lab: Brenda Schulman
• Publication: Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.

Backbone

• Vector backbone: pGEX
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cul3 N-terminal domain
• Alt name: Cul3
• Alt name: cullin 3
• Species: H. sapiens (human)
• Mutation: contains aa 1-309
• Entrez Gene: CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
• Promoter: tac
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pGEX5'

Gene/Insert 2

• Gene/Insert name: Cul3 C-terminal domain
• Alt name: Cul3
• Alt name: cullin3
• Species: H. sapiens (human)
• Mutation: contains aa 310-768
• Entrez Gene: CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
• Promoter: IRES
• Tag / Fusion Protein:
  • IRES (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: none

Gene/Insert 3

• Gene/Insert name: RBX1
• Alt name: ring-box 1
• Species: M. musculus (mouse)
• Mutation: contains aa 5-108
• Entrez Gene: Rbx1 (a.k.a. 1500002P15Rik, ROC1)
• Promoter: IRES
• Tag / Fusion Protein:
  • IRES (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: none
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry