pHnCBS1D
(Plasmid
#52065)
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Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNS3
- Backbone size w/o insert (bp) 4891
- Total vector size (bp) 13242
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
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SpeciesH. neapolitanus
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Insert Size (bp)8351
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHnCBS1D was a gift from David Savage (Addgene plasmid # 52065 ; http://n2t.net/addgene:52065 ; RRID:Addgene_52065) -
For your References section:
Modularity of a carbon-fixing protein organelle. Bonacci W, Teng PK, Afonso B, Niederholtmeyer H, Grob P, Silver PA, Savage DF. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):478-83. doi: 10.1073/pnas.1108557109. Epub 2011 Dec 19. 10.1073/pnas.1108557109 PubMed 22184212
Map uploaded by the depositor.
