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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLin28B
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SpeciesH. sapiens (human)
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Insert Size (bp)753
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Entrez GeneLIN28B (a.k.a. CSDD2)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CAATGGGCGTGGATAGCG
- 3′ sequencing primer CCAGGGTCAAGGAAGGCAC (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG-Lin28B was a gift from Narry Kim (Addgene plasmid # 51373 ; http://n2t.net/addgene:51373 ; RRID:Addgene_51373) -
For your References section:
Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Heo I, Joo C, Cho J, Ha M, Han J, Kim VN. Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014. 10.1016/j.molcel.2008.09.014 PubMed 18951094