pENTR-p27(kip1)
(Plasmid
#50026)
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PurposepENTR vector for human cyclin-dependent kinase inhibitor 1B
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2549
- Total vector size (bp) 3146
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep27(kip1)
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Alt namecyclin-dependent kinase inhibitor 1B
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Alt nameCDKN1B
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SpeciesH. sapiens (human)
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Insert Size (bp)597
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GenBank IDNM_004064
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Entrez GeneCDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13_F
- 3′ sequencing primer M13_R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-p27(kip1) was a gift from Jamie Davies (Addgene plasmid # 50026 ; http://n2t.net/addgene:50026 ; RRID:Addgene_50026) -
For your References section:
A library of mammalian effector modules for synthetic morphology. Cachat E, Liu W, Hohenstein P, Davies JA. J Biol Eng. 2014 Nov 19;8(1):26. eCollection 2014. 158 [pii] PubMed 25478005
Map uploaded by the depositor.