pGEX-4T-1 3xFlag-p38alpha
(Plasmid
#47575)
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PurposeBacterial expression plasmid for GST-3xFlag-tagged p38alpha
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5053
- Total vector size (bp) 6136
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Modifications to backbone3xFlag tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep38alpha
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Alt nameMapk14
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Alt namep38
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SpeciesM. musculus (mouse)
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Insert Size (bp)1083
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GenBank IDNM_011951
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Entrez GeneMapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- 3xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #20351.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1 3xFlag-p38alpha was a gift from Kevin Janes (Addgene plasmid # 47575 ; http://n2t.net/addgene:47575 ; RRID:Addgene_47575) -
For your References section:
A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447