pBp-FGFR2b-T787K
(Plasmid
#45709)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 45709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBabe puro Gateway
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7900
-
Modifications to backboneGateway cloning sequences flank region with gene insert for use with the Gateway system reagents.
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFGFR2 IIIb
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2400
-
MutationT787K
-
Entrez GeneFGFR2 (a.k.a. BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Gateway reagents
- 3′ sequencing primer Gateway reagents (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBp-FGFR2b-T787K was a gift from Matthew Meyerson (Addgene plasmid # 45709 ; http://n2t.net/addgene:45709 ; RRID:Addgene_45709) -
For your References section:
Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong KK, Haddad RI, Meyerson M, Hammerman PS. Cancer Res. 2013 Jun 20. 10.1158/0008-5472.CAN-12-3950 PubMed 23786770