pBbA5k-EPL122
(Plasmid
#45440)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbA5k
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH1 with AcrAB knocked out
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameacrF
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SpeciesSynthetic
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Insert Size (bp)4274
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GenBank IDNP_417732.1
- Promoter LacUV5
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG ACG AAA CAT GCC AGG TTT TTC C
- 3′ sequencing primer CAG TGG TGA TGG TGA TGA TGT CCT TTA AAG CAA CGG CGG ATC ACC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbA5k-EPL122 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 45440 ; http://n2t.net/addgene:45440 ; RRID:Addgene_45440) -
For your References section:
Engineering microbial biofuel tolerance and export using efflux pumps. Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi MZ, Mukhopadhyay A. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. 10.1038/msb.2011.21 PubMed 21556065