pCFJ1000
(Plasmid
#44475)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDESTR4-R3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4070
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Vector typeGateway Destination vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMinimal Mos1, PuroR, peel-1, [4-3] Gateway
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SpeciesD. melanogaster (fly), C. elegans (nematode); Bacterial
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Insert Size (bp)3761
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13-fwd20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ1000 was a gift from Erik Jorgensen (Addgene plasmid # 44475 ; http://n2t.net/addgene:44475 ; RRID:Addgene_44475) -
For your References section:
Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM. Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16. 10.1038/nmeth.2889 PubMed 24820376