pHEX2 T494 TALENs
(Plasmid
#44464)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHEX2
- Backbone size w/o insert (bp) 13812
- Total vector size (bp) 21391
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Vector typePlant Transformation
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtCRU3 T494 TALENs
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Alt nameArabidopsis thaliana CRUCIFERIN 3 gene Target 494 TALENs
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SpeciesSynthetic
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Insert Size (bp)7549
- Promoter CaMV 35S
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer RAJ-417 (CAACCACGTCTTCAAAGCAAGTG)
- 3′ sequencing primer m13 Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe gene was custom-made, but includes sequence from pZHY013 from Zhang et al. 2012
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHEX2 T494 TALENs was a gift from Roger Hellens & Ross Johnson (Addgene plasmid # 44464 ; http://n2t.net/addgene:44464 ; RRID:Addgene_44464)
Map uploaded by the depositor.