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Addgene

pCAG HRP-TM
(Plasmid #44441)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44441 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG
  • Total vector size (bp) 5508
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Horseradish peroxidase fused to the transmembrane domain of PDGFR
  • Species
    Synthetic
  • Insert Size (bp)
    1224
  • Promoter CAG
  • Tags / Fusion Proteins
    • HA tag (N terminal on insert)
    • myc tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct has the Ig k-chain leader sequence on the N-terminus, which directs the protein to the secretory pathway. HRP faces the extracellular side of the plasma membrane.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG HRP-TM was a gift from Alice Ting (Addgene plasmid # 44441 ; http://n2t.net/addgene:44441 ; RRID:Addgene_44441)
  • For your References section:

    Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Science. 2013 Jan 31. 10.1126/science.1230593 PubMed 23371551