pBH6119-toxTpro-gfp
(Plasmid
#44388)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBH6119
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Vector typeBacterial Expression
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Selectable markersthy+
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametoxTpro
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Alt nametoxT promoter
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Alt name(-172) to (+45) toxT promoter fragment
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SpeciesVibrio cholerae
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Insert Size (bp)216
- Promoter toxT
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GTA TAG CAA AGC ATA TTC AG
- 3′ sequencing primer AAA TAA ACG CAG AGA GC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe pBH6119 plasmid was obtained from Michele Swanson's lab, at the University of Michigan.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The toxT promoter drives expression of a stable form of GFPmut3. Please note that although this plasmid can be propagated in E. coli, it is a Vibrio cholerae vector and should be used in a Vibrio strain for experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBH6119-toxTpro-gfp was a gift from Victor DiRita (Addgene plasmid # 44388 ; http://n2t.net/addgene:44388 ; RRID:Addgene_44388) -
For your References section:
Small-molecule inhibitors of toxT expression in Vibrio cholerae. Anthouard R, DiRita VJ. MBio. 2013 Aug 6;4(4). pii: e00403-13. doi: 10.1128/mBio.00403-13. 10.1128/mBio.00403-13 PubMed 23919997